Intraperitoneal Administration of Short-Chain Fatty Acids Improves Lipid Metabolism of Long–Evans Rats in a Sex-Specific Manner

Short-chain fatty acids (SCFAs) are microbial metabolites, mainly generated by the action of gut microbiota on dietary fibers. Acetate, propionate, and butyrate three main SCFAs produced typically in a 60:20:20 molar ratio colon. butyrate, when given individually as supplements, have shown protective role obesity hyperglycemia; however, sex-specific effects mixture SCFAs, ratio, regulation lipid metabolism profile not known. Male female Long–Evans rats were (acetate, butyrate; 60:20:20) each day for seven days intraperitoneally; plasma hepatic lipids, gene expression, lipidomics analyzed. significantly decreased triglycerides cholesterol males, whereas acyl composition cholesteryl esters, triglycerides, phospholipids was modulated females. mRNA expression acetyl-CoA carboxylase-1 both males Our findings demonstrate first time that (60:20:20) improved levels manner may provide cardio-protective anti-inflammatory sexes, via independent mechanisms.